Fecha/hora: 01/06/2024 14:16:54

Biblioteca(s): | INIA Las Brujas.
Fecha corriente: | 09/11/2017
Fecha de la última actualización: | 12/12/2018
Autoria: | FEDERICI, M.; BAJSA, N.; LAGURARA, P.; REVALE, S.; MARCONDES, J.A.; DALLA RIZZA, M.
Afiliación: | MARIA TERESA FEDERICI RODRIGUEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; NATALIA BAJSA VALVERDE, MEC/ IIBCE (Instituto de Investigaciones de Ciencias Biológicas "Clemente Estable"); PAULA LAGURARA, MEC/ IIBCE (Instituto de Investigaciones de Ciencias Biológicas "Clemente Estable"); SANTIAGO REVALE, INDEAR (Instituto de Agrobiotecnología de Rosario); JACKSON A. MARCONDES, Universidad Estadual Paulista (UNESP)/ Campus de Jaboticabal; MARCO DALLA RIZZA VILARO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay.
Título: | Soil and rhizosphere bacterial diversity in maize agro-ecosystem.
Año de publicación: | 2017
Fuente/Imprenta: | Sustainable Agriculture Research, 2017, v.6 (3): 35-51.
ISSN: | 1927-050X (Print) // 1927-0518 (Online)
DOI: | 10.5539/sar.v6n3p
Idioma: | Inglés
Notas: | Article history: Received: April 3, 2017; Accepted: May 20, 2017; Online Published: June 16, 2017.

Contenido:

ABSTRACT.
Management practices used in maize production have an impact on soil agro- ecosystems where different microbial communities coexist. Soil inhabiting bacteria are numerous and diverse, but we know very little about their ecological distribution. Here we analyzed the bacterial community diversity in the rhizosphere of two transgenic maize cultivars, in agricultural soil before sowing and in non-cultivated soil in an experimental site in the south region of Uruguay. We followed two culture-independent methods: DGGE (denaturing gradient gel electrophoresis) and 454-pyrosequencing of 16S rRNA gene amplicon. Through pyrosequencing, the three environments analyzed presented differences in terms of bacterial composition. However, no differences were found in the relative abundance of the ten most represented phyla in the rhizosphere of the two cultivars at different phenological stages. We found significant differences of Bacteroidetes, Gemmatimonadetes, Planctomycetes, Proteobacteria and Verrucomicrobia phyla when comparing agricultural and non-cultivated soils, as well as a significant enrichment of members of the phylum Gemmatimonadetes in all rhizosphere samples compared to soil. Through DGGE analysis we evidenced that maize rhizosphere bacterial communities changed at different phenological stages in both cultivars. We also provided baseline information about bacterial specific taxa within maize agro- ecosystem for further evaluation of possible rhizosphere bacterial community shifts of genetically modified maize cultivars under different management practices.
Copyright © Canadian Center of Science and Education

Palabras-Clave: | DGGE; METAGENOMICS; PYROSEQUENCING; RHIZOSPHERE; SOIL BACTERIA; TRANSGENIC MAIZE.
Categoría del tema: | --

URL:

http://www.ainfo.inia.uy/digital/bitstream/item/7925/1/67345-252782-1-PB.pdf
http://www.ccsenet.org/journal/index.php/sar/article/view/67345/37668

Registro original: | INIA Las Brujas (LB)
Ejemplares: | LB - (101493-1) - PXI - AP - DD - PP/SUSTAINABLE AGRICULTURE RESEARCH/2017 -  -  - DIS